Microscopic examination of the skin specimens of control animals on day 21 of observation showed the signs of purulent burn wound (Fig. 3).
Specimens of control and HA-Zn coated groups were evaluated by a surface roughness profilometer tester (Surftest SJ-210, Mitutoyo Corporation, Tokyo, Japan,) according to ISO 4287-1997 [24] with a diamond tip radius of 5 μm, a scanning speed 0.5 mm/s, a resolution of 0.01 μm, a Gaussian filter, and a cut-off length of 8 mm.
The morphological examination of specimens of control group (with occlusion of middle cerebral artery and without treatment) revealed an expressed perivascular and pericellular edema of brain tissue.
The expression levels of certain transcripts, including RANTES, aggrecan, collagen type 11, and fibromodulin, may reflect smaller amounts of epithelium and greater amounts of cartilage in biopsy specimens of control subjects compared to those of asthmatics.
In specimens of control animals and many of those of 1 week experimental animals, on the other hand, the blood vessel walls showed generally moderate or weak reactions but could also be seen to be unreactive.
Twenty specimens of "control bile" were obtained from the patients with cholesterol gallbladder polyps receiving surgical treatment, as it is practically infeasible to obtain pure normal bile in clinical practice.
Total RNA was extracted from frozen liver specimens of control and fibrotic rats (RNeasy Kit; Qiagen, Hilden, Germany), then 1 μg of total RNA was reverse transcribed using a complementary DNA synthesis kit (High-Capacity cDNA Reverse Transcription Kit; Applied Biosystems, Foster City, CA, USA).
Western blot analysis of total protein extracts from CD ileal specimens taken in involved areas and of controls showed a strong expression of Gp96 in five out of six CD ileal mucosa specimens and only one out of six ileal mucosa specimens of controls (figure 1D).
The comparison of effective bond lengths of exposed specimens with those of control specimens are presented later in this paper to investigate the effect of environmental conditions on the effective bond length.
Based on the comparison of experimental results of exposed specimens to control specimens in terms of bond strengths, failure modes and strain profiles, the most significant degradation of pull-out strength was observed in specimens exposed to outdoor environment, whereas temperature cycles did not cause any deterioration of strength.
