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Laboratory culture work requires biosafety-level 3 (BSL-3) conditions and primary cultivation from a clinical specimen may require a seven-day incubation before colonies visible to the naked eye appear.
Furthermore, obtaining a positive specimen may require invasive techniques.
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When collected through primary care physicians, billing issues can be problematic, and each home visit for collection of blood specimens may require several hours of staff time.
Appropriate investigation is limited by the diagnostic techniques available; virus isolation from cerebrospinal fluid or the brain can delay a diagnosis because culturing is slow, sensitivity may be poor, and obtaining specimens may require special techniques.
In the absence of clinical response, the diagnosis may require surgical pathology specimens.
In conclusion, the identification of all organisms involved in polymicrobial invasive infections may require cultures of specimens from all accessible septic foci.
Determination of the true accuracy of fibrosis imaging may require histologic evaluation of larger specimens than those obtained by percutaneous biopsy.
These specimens can be processed in sentinel site laboratories, but further analyses may require their transport to additional laboratories.
That may require legislation.
Cloning may require some horsepower.
Markets may require "adult supervision".
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