Such testing is commonly performed indirectly, in that the pure bacterial culture or isolate grown from the original patient specimen is re-inoculated into drug-containing media.
The specimen was re-anodized for long periods to examine the growth of porous anodic oxide films at the area where substrate had been exposed by measuring current variations and morphological changes in the oxide during the re-anodizing.
The specimen was re-discovered in a cabinet in the herbarium of the Royal Horticultural Society five years ago.
The pellet obtained from the rinse specimen was re-suspended in 1 ml of sterile phosphate buffered saline.
The specimen was re-probed using an anti-GAPDH antibody (Chemicon, Watford, UK), which served as loading control.
Modified radical mastectomy was performed in all patients three weeks after the last cycle and the specimen were re-evaluated for any change in the bcl-2/bax ratio.
Of the remaining 105 patients, the histopathological slides of a biopsy-, core-, or hysterectomy specimen were re-examined independently, by three experienced gynaecopathologists (JB, AWvT, and MM).
The diagnosis of psoriasis was histologically confirmed in all cases and each specimen was re-evaluated by three pathologists (D.T., M.V. and B.K .. None of the patients had received any topical treatment for at least 1 month prior the study.
When the diagnoses were not in agreement, the biopsy specimen was re-examined and discussed until a consensus was reached.> The tissue sections were deparaffinized, immersed in 10 mM citrate buffer (pH 6.0) and heated in a microwave oven for 15 min for antigen retrieval.
This right flank basal cell carcinoma specimen was re-examined and immunohistochemical stains were performed following discovery of the lung metastases; the tumor cells were negative for synaptophysin, chromogranin, and cytokeratin 20 by immunohistochemistry, findings which argue against the possibility of a cutaneous neuroendocrine carcinoma (Merkel cell carcinoma),.
The specimens were re-tested in parallel by m-RT-PCR-ELISA versus the gold standard culture and immunfluorescence, and versus individual RT-PCR.
