Previous studies had long indicated that plating efficiencies and derivation of primary cultures from clinical lung cancer specimens is poor, and is dependent both on culture conditions and host-related factors [8], [9], [10], [11].
Vibration work was performed but the compacting ability of NC specimens was poor.
: PCR assays were not available for these patients because no fresh specimens were sampled and the quality of the DNA extracted from the paraffin-embedded specimens was poor, not allowing a conclusive diagnosis.
However, the holotype specimen is in poor condition, and recollected specimens were reported to have amyloid basidiospores (Tanaka and Hongo 2003).
The family is an ancient group of small species which is thought to have diverged early from the rest of the tubenoses; the supporting fossil record is poor, with specimens from California dating back only to the Late Miocene (11.6 5.3 million years ago).
Therefore, direct triage testing on self-sampled material is preferred over an extra visit to a physician, but the concordance between cytology on self-obtained specimens vs physician-taken samples is poor (Nobbenhuis et al, 2002; Garcia et al, 2003; Brink et al, 2006; Snijders et al, 2013).
2 In addition to the challenges associated with sampling of appropriate SF specimens, the reliability of polarising microscopy is poor.
DNA was extracted from the samples collected by Proshek et al., Powell, and Davenport (sources #1, 4, 5, Additional file 1: Table S8) from two legs (or leg fragments and antennae, if the specimen was in poor condition) using the DNeasy Tissue Extraction Kit (Qiagen, Valencia, CA).
One of the main problems arising in confocal imaging of thick specimens is the poor penetration of the antibodies within the whole structure.
As cattle, the are likely to be poor specimens - and diseased, as wel but this does not matter to the Masai, who use them as currency, even for buying a bride, and it is the numbe, not the condition of the animals that counts.
