Next, the specimens were manually polished using 600, 1200 and 2000 grit sandpaper (Norton S.A., São Paulo, SP, Brazil) and subjected to hardness test using a Hardness Tester HMV 2000 (Shimadzu, Tokyo, Japan) at automatic procedure with a load of 50 g applied for 15 s.
To investigate 19 bladder tumours in aCGH analysis, tumour specimens were manually microdissected and DNA was isolated using the QIAamp DNA Mini Kit (Qiagen, Hilden/Germany) according to manufacturer's protocol.
8 Briefly, muscle biopsy specimens were manually homogenized in phosphate-buffered saline containing 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 1% protease inhibitor cocktail, and 1% phosphatase inhibitor cocktail.
Fifty seven fields per patient, including demographics, preoperative prostate-specific antigen, pathological Gleason score and stage and other pathological data relating to the prostatectomy specimens were manually entered in a non-relational database (Microsoft Excel spreadsheet) for the first 853 of these cases (2004 2008).
Total specimen area was manually segmented, and the blue-stained pixels (representing collagen) were segmented using manual intensity thresholding.
It is manually woven into the towel.
CISMeF is manually maintained.
Of these seven specimens, one was manually selected for less frequent codon 12 mutation.
The first 200 specimens encountered were manually separated and mounted on cardboard slides in a standard orientation (edge on, aperture facing).
Also, gross sectioning of prostate specimen was performed manually in almost half of the subjects, which is technically extremely difficult to be done at 4-mm intervals thereby introducing errors [22].
