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In such tests, the load on a steel specimen is maintained constant while its temperature is increased at a given rate and the changes in gauge length are constantly recorded.
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For each heating run, the specimen was maintained at the destination temperature for 15 min and then cooled to room temperature using a fan.
For each test, the specimen was maintained at a first rate temperature of 60″ and the final one of 30″.
Primary fibroblast isolated from clinical specimen were maintained in Dulbecco's Modified Eagle's Medium/F-12 Nutrient Mixture (DMEM/F-12) (Invitrogen) supplemented with 10% fetal bovine serum (Industrial Biological), 0.1 mM non-essential amino acids and 1 mM sodium pyruvate (Invitrogen, above).
The specimen was maintained during 10 min in these conditions.
The coverslips were then washed and placed in Leiden coverslip chambers, adapted to the microscope, where the temperature of the specimen was maintained in 35-37°C 35-37°C
Therefore, mixing cores and resection specimens in a single study is likely to suffer from issues of variability between types of specimens but still their data suggest that the relative expression levels within each type of specimen are maintained.
Cells were incubated for 5 h in the transfection mixture at 37°C, placed in Leiden coverslip chambers and adapted to the microscope, where the temperature of the specimen was maintained at 35-37°C 35-37°C
or thermal exposure, it is imperative to insure environmental protection during the entire transfer process so that all specimens are maintained in a pristine state before, during, and after analysis.
Specimens are maintained in liquid nitrogen until analysis.
The specimens were maintained in aerated tanks for two weeks.
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