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The un-cracked ligament of the tubular specimen is fixed in y-direction (but it can move in x- and z-directions) considering the symmetry condition.
The specimen is fixed and permeabilized in methanol, then protein deposits in the specimen are selectively stained using antibodies or specific fluorescent dyes.
After the specimen is fixed, the segments of bowel including the tumor, the 30 mm segment of intestine proximally and distally to the tumor, and the attached mesentery were sectioned transversely at 3 4 mm intervals with a sharp knife to produce slices that included the tumor, the adjacent lymph nodes, and the serosal and nonperitonealized resection margins 16.
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The specimen was fixed in 10% formalin, and paraffin sections were prepared.
One antral specimen was fixed in formol solution at 10% and embedded in paraffin.
Each specimen was fixed in 10% formaldehyde and embedded in paraffin.
Each kidney specimen was fixed in 4 % paraformaldehyde (PFA) for 24 h and paraffin embedded.
For histological examination, the specimen was fixed in 4% paraformaldehyde, embedded in paraffin, and sectioned.
Kidney specimens were fixed in 0.1 mol/L phosphate-buffered Karnovsky's fixative.
Specimens were fixed again overnight at 4 °C.
All specimens were fixed in paraformaldehyde and embedded in paraffin.
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CEO of Professional Science Editing for Scientists @ prosciediting.com