As the opening of the biopsy needle (10 mm) was larger than the target lesion (5 mm), and the needle itself has a 2-mm forerun (Fig. 1, top), only the distal part of the actual specimen was expected to hold pathological tissue, which was confirmed by visible 5-ALA-induced protoporphyrin IX (PpIX) fluorescence only within the tumor tissue (Fig. 1, bottom).
Cryo-electron tomography has made particularly impressive gains during the last five years, and its application to frozen-hydrated specimens is expected to grow in the near future, despite the low tolerance of frozen-hydrated specimens to electron exposure.
The analysis of clinical specimens is expected to provide expression patterns that may allow to identify which patients would respond to intravesical or systemic treatments, or present a higher progression risk to develop invasive or metastatic disease.
All species used in this study engage in symbiotic associations with intracellular dinoflagellate symbionts; therefore, RNA extracted from these specimens is expected to include contributions from both animal hosts and dinoflagellate symbionts.
Although BP1600 and CP1600-1 specimens were expected to show high strain in a central part, their strain distributions are uniformly formed in a certain region of a central part.
Smear positive specimens are expected to be culture positive so yields are presented mainly as a comparison to the smear negative SE.
During weeks in which >15% of surveillance specimens are expected to be positive for influenza, testing and empirical treatment might be indicated in any patient with respiratory symptoms.
Thus, examination for the presence of MMP-7 in tumour biopsy specimens before treatment is expected to be useful for decision making about the treatment course and to predict prognosis.
Because of the 3D geometry of the specimen, the transverse beam is expected to gain plastic capacity with higher vertical displacements.
The increasing availability of genomic data, particularly for rare taxa and museum specimens [ 47, 85], is expected to help resolve these evolutionary uncertainties.
