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The images are created after a specimen is embedded in gelatin, sliced into microscopic sections and digitized.
The SNR and contrast of an electron micrograph also depend on the thickness of the ice in which the specimen is embedded.
To overcome incomplete stain embedding, Frank and colleagues have developed a carbon sandwich technique, in which the specimen is embedded in a continuous layer of stain in between two carbon films.
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After dehydration, each specimen was embedded by infiltration in Spurr's medium.
Each specimen was embedded in paraffin and cut into 3 µm serial sections, stained with hematoxylin eosin and subjected to histopathological examination by light microscopy.
Thereafter, each specimen was embedded in paraffin.
The biopsy specimen was embedded non-decalcified into methylmethacrylate.
Specimen were embedded in tissue-tek and quick-frozen in fluid nitrate.
Each specimen was embedded in Epon812 for transmission electron microscopy (TEM).
The obtained specimen was embedded into OCT (Optimal Cutting Temperature) compound and immediately frozen in liquid N2.
After dehydration through gradient ethanol solutions, the specimens were embedded in Araldite-Epon (Embed-812, Electron Microscopy Sciences, USA).
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