Each specimen was carefully aligned with the grooves either parallel or perpendicular to the indentor motion direction, as illustrated in Fig. 1a, with the assistance of the attached microscope.
Fracture in the uniaxial test is always explosive, but when the apparatus and specimen are carefully prepared, such explosive fracture accompanies faulting planes in the specimen (Yanagidani et al.,1985), which is the key technical aspect in our study.
The specimen was carefully homogenized with a Pasteur pipette for one minute, followed by vortexing for 30 sec–1 minute, to ensure uniform distribution of bacilli.
A small film specimen was carefully deposited onto carbon tabs (Agar Scientific, Stansted, UK) and coated with carbon (Agar turbo carbon coater) to improve conductivity.
The specimen was carefully cleaned from adipose tissue, cut into small pieces, and homogenized in 2 ml lysis buffer (4% SDS in 0.1 M Tris HCl, pH 7.6) with an ULTRA-TURRAX homogenizer.
Each tumor specimen was carefully examined after removal from the patient, and we found that most of the hepatic NPC metastases displayed an infiltrating growth pattern with a deficiency of blood supply and lack of a capsule.
Specimens were carefully selected from women with conditions unlikely to affect the microvascular networks.
We developed a large research team and ensured that tumour specimens were carefully obtained and annotated in our tissue repository.
The geometric and material properties of the continuous beam test specimens were carefully recorded and supplemented by tests on simply supported specimens of the same cross-sections.
Specimens were carefully prepared for analysis by the curators of the Institute Carlos III in Madrid, the repository of the SH fossils, removing any consolidants previously applied to the bone surfaces.
