LMS contributed to microbiological analysis of the isolate and handling of patient specimen in case 2 and helped with revision of the manuscript.
Tumor allocation was assessed either by the primary endoscopy report including endoscopic ultrasound or by the pathological report of the resected specimen in case of gastrectomy.
Histopathology was performed also on the entire tumour specimen (in case of limb amputation or limb sparing using an allograft to substitute the tumour segment) or on a sample of the tumour (in case of limb sparing using the pasteurized neoplastic autograft), in order to confirm the diagnosis [ 73, 74].
Thus, we do not have any specific analysis of multifocality and multicentricity, but we have the notion of 'residual tumour' on the specimen in case of multiple surgery, maximal tumour size and final margin status to predict the best surgical option (especially mastectomy).
All suspect mucosal lesions were eventually resected completely through endoscopically assisted surgery or a biopsy of a substantial portion of the lesion was performed to obtain a representative specimen in case it was not possible to make the diagnosis and treatment during the same session.
The HSV HRM assay provides a cost-effective alternative method to conventional more expensive assays and can be used in routine clinical specimens, in cases where it is particularly necessary to detect and distinguish HSV-1 from -2.
We used endoscopic biopsy specimens in cases of initially metastatic patients, whereas resected samples were used in cases of recurrence after curative resection.
The difference in gestational age between baseline and predelivery specimens in cases and controls did not differ significantly (154 v 148 days, P=0.08).
The indices of renal sclerosis, which are higher in second biopsy specimens in cases of persistent IgA nephropathy as compared to the first, are higher when there are more macrophages producing MRP8 than when there are less.
The laboratory diagnosis of Q fever depends on the stage of disease (acute or chronic), which in turn determines which sample should be used for analysis: blood, cerebrospinal fluid, bone marrow, cardiac valve biopsy, vascular aneurysm or graft, bone biopsy, liver biopsy, milk, placenta, fetal specimens in cases of abortion, or cell culture supernatants [ 38].
