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A specimen harboring a single allele at each of the four genomic regions is presumably infected with a single P. jirovecii type, and each combination of four simple SSCP patterns defines a type.
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Gonzalgo and co-workers also found relatively high levels of methylation in normal renal tissue, collected from specimens harboring a renal neoplasm [ 17].
This non-significant finding may be attributed to the low number colon tumor specimens (17 of 81 specimens) harboring an ERCC1-19q13 gain.
Due to the nature of the ERCC1-19q13/CEN-2 FitH probe, is is unknown to what extent other 19q genes are gained in specimens harboring an ERCC1-19q13 gain.
If a specimen harbors two alleles of one or more of the four genomic regions, the patient was considered coinfected with two or more P. jirovecii types (25 ).
The use of a ratio cut-off of 1.5, reflecting an additional gene copy in a diploid cell, classified 41 (27.0%) specimens as harboring an ERCC1-19q13 gain.
Of the 15 samples of ECG1 that contained mutation, 6 (40%) cases harbored more than one mutation (one specimen harbored 2 mutations in one exon).
Analysis of 15 masked specimens harboring clinically reported variants yielded concordant calls for 13/13 variants at AF of ≥15%.
Samples were collected from 60 women who had cervical cytology specimens harboring cervical intraepithelial neoplasia (CIN) 2 or 3.
We stained 27 breast specimens harboring abundant LCIS with antibodies to smooth muscle myosin heavy chain, smooth muscle actin, and calponin.
Among a cohort of 61 sporadic colorectal cancer specimens we found that 4/33 (12.1%) specimens harboring KRAS mutations were incorrectly classified as KRAS-WT using regular (conventional) PCR melting curve analysis and Sanger sequencing, despite dissection of samples for tumor enrichment.
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