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The specimen grid is carried in a small holder in a movable specimen stage.
The particles were then transferred by an ultrasonic treatment into a solution and further transferred to a microscopic specimen grid.
Diluted suspensions and nanoemulsions were deposited on specimen grid (Formvar-Carbon support films, Electron Microscopy Sciences), negatively stained with uranyl acetate solution (2% w/v) [37] and observed at different magnifications.
A carbon-coated 200-mesh copper specimen grid was glow-discharged for 1.5 min. One drop of nanoparticle suspension was deposited on the grid and allowed to stand for 1.5 min after which any excess fluid was removed with filter paper.
About 3 μl of aliquots (∼20 μg protein/ml in 50 mmol/L Tris, pH 7.6, 100 mmol/L ammonium sulfate, 10% (v/v) glycerol, 1 mmol/L EDTA, 10 μmol/L ZnSO4, 0.02% NP-40, 10 mmol/β-ME) was applied to a carbon-coated 400-mesh Cu EM specimen grid freshly glow discharged and was then preserved by staining with 0.75 % (w/w) uranyl formate solution.
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EV's were fixed with 1% buffered glutaraldehyde and adsorbed onto carbon-formvar coated specimen grids before immunolabelling.
The EV suspension was placed on the gold-loaded, epoxy-embedded specimen grids and in 1% glutaraldehyde, postfixated in 1% OsO4 and stained with 1% aqueous uranyl acetate.
The substrate was prepared by adsorbing 15 nm gold particles (Department cell biology, University of Utrecht, the Netherlands) on formvar/carbon coated copper specimen grids and then embedded the gold-coated grids in a thin layer of epoxy resin between two layers of Aclar film and polymerized at 60 °C for 48 hours.
The grids were brought from Ted Pella, Inc.; 300 mesh copper specimen grids with Formvar/Carbon support films.
Novel specimen grids (Rhinow and Kühlbrandt 2008; Pantelic et al. 2010; Yoshioka et al. 2010; Russo and Passmore 2016) made with materials that more readily dissipate charge and heat during imaging can minimize beam-induced specimen motions as well as the bubbling and distortions caused by radiation damage.
Sections were mounted on Formvar/carbon-coated metal specimen grids.
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