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Thus, a specimen found to be positive for any of the 7 antigens in the assay was considered to be positive.
The Genetic Systems HIV-1 Western Blot (Bio-Rad used was used to confirm the status of any specimen found to be diagnostically indeterminate after EIA testing.
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Nevertheless, when the proportion of specimens found to be infected on follow-up were removed, pregnant women were not more likely to have false-positive HIV EIA test results than others testing for HIV, as previously thought.
Firstly, we used the National Cancer Institute definition: percentage of specimens found to have no cervical intraepithelial neoplasia/grade I disease, 31 and, secondly, we used the NHS cervical screening programme definition: percentage of specimens found to have no cervical intraepithelial neoplasia.
All specimens found to have an absorbance level less than the cut-off value were considered negative.
Specimens found to be negative were subjected to RT-PCRs with alphavirus and flavivirus generic primers and West Nile virus specific and SHUV-specific primers (6, 7 ).
In the current study, the percentage of CRC specimens found to be positive for CDw75 was higher in tumour CRC advanced stages.
All specimens found to be negative by real-time PCR possessed exponential amplification curves for internal control PCR (data not shown).
One study (M) used a postpandemic, cross-sectional design with retrospective assessment of prepandemic titers for those specimens found to be seropositive.
However, specimens found to be positive for HPV DNA by nested PCR and confirmed by DNA sequencing were considered sufficient even when no β-globin gene amplification was observed.
Conversely, taking into account all of those specimens found to be positive by MT-PCR in this study, IF detected a significant pathogen in 56/73 (77%) and in-house RT-PCRs detected 87/88 999%).
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