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Investigators in the field need to collect adequate specimens for virus isolation.
In parallel with human epidemiologic studies, visits were made to Durba in May and October 1999 to collect specimens for virus ecostudies.
Specimens for virus isolation were collected from outbreaks in different districts of Uganda, sentinel sites, and Mulago hospital, the national teaching and referral hospital, in Kampala from 2000 to 2002.
As part of routine measles case investigations, urine samples and throat swab specimens for virus isolation were collected along with serum samples (0 12 days after rash onset) from patients across Uganda during 2006 through 2009.
Prior to specimen inoculation for virus isolation, 200 μl of the specimen was transferred to a 1.5 ml microtube and stocked at -80°C until application to real-time RT-PCR assay.
Other factors that differ among studies are the age and preexisting medical conditions of study participants and the timing of specimen collections for virus testing.
The etiologic agent was confirmed to be Japanese encephalitis virus by analyzing 326 acute-phase clinical specimens for virus-specific antibodies and viral RNA and by virus isolation.
The plasma specimen was processed for virus isolation by embryonated egg injection, according to the standard protocol described by Harmon (3 ).
Wild-type rotavirus strains were detected in 8 (12%) of the 65 specimens with any virus detected, whereas a genotype could not be determined for the remaining 57 (88%) specimens for which virus was detected by qRT-PCR.
Study design: A total of 145 patient specimens for influenza virus detection submitted in either viral transport medium or in sterile containers were evaluated by the three methods.
In 1998, there were in total 19,848 specimens for influenza virus isolations collected from all public hospitals (covering about 95% of all hospital beds) and outpatient clinics in the whole territory of Hong Kong SAR.
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