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We analyzed the proximal 3-cm length of the maternal hair specimen for total mercury content.
Monitoring of response was performed after each treatment cycle and at study-end by means of quantification of serum immunoglobulins, serum protein electrophoresis, immunofixation and collection of 24-hr urine specimen for total protein, electrophoresis and immunofixation.
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200 μl of each dilution of saline and the corresponding blood specimen was used for total DNA extraction (as described above).
To determine the compression strength of the block filled with grouted concrete, the prism test was conducted, where there were every three prism specimens for a total of eight parameters (two grouted concrete parameters and four single block parameters; total of 24 specimens).
For squid, we measured eye diameter (see above) and body mass in fixed specimens for a total of 58 species.
Subsequent PCR testing at CDC identified >1 virus in 53 (9.3%) specimens, for a total of 88 (15.4%) specimens with a detected pathogen (includes 5 specimens previously positive for rotavirus by EIA and 2 specimens positive for bacteria and virus).
We measured the largest exposed eye diameter and body mass in fixed specimens for a total of 237 acanthomorph species representing 237 families, a phylogenetically and ecologically very broad sample (Additional file 3).
The specimens were used for total blood cell counts and measurement of serum concentrations of electrolytes, creatinine, alanine aminotransferase (ALT), and glucose.
Serum specimens were tested for total virus-specific antibodies with an indirect enzyme immunoassay (EIA) with SARS-CoV lysate as the antigen (9 ).
A subset of 30 specimens was used for total DNA extraction with the MoBio PowerPlant Pro DNA Isolation Kit (MO BIO Laboratories, Carlsbad, California, USA).
Tissue specimens were prepared for total RNA extraction, amplified, and hybridised to a high-density oligonucleotide microarray, and the 5-year probability of metastatic risk was computed for each of the patient cases based on the 'locked' 22-marker genomic classifier, as previously described [ 13].
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