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Images of five random fields were taken from each specimen for quantitative analysis.
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The tissue specimens for quantitative RT PCR were from 23 BC patients who had received cystectomy or TUR-BT at Kagoshima University Hospital between 2006 and 2009.
Animals were killed at 4, 12 and 24 weeks, and specimens processed for quantitative histological examination.
Of the additional tumour specimens selected for quantitative PCR analysis (13 TURP, 13 radical prostatectomy), 14 were poorly, 11 moderately and one well differentiated.
Using cutaneous swab specimens from patients for quantitative PCR (qPCR) testing rather than cutaneous biopsy specimens is a major innovation in the diagnosis of SFG rickettsioses (2 – 4 ).
A subset of precisely measured specimens was analysed for quantitative assessment of the observed trends.
All the serum specimens were tested for quantitative anti-HBs antibodies using a commercial test kit based on the immunoenzimatic method MEIA (AxSYM AUSAB; Abbott Laboratories, Abbott Park, IL USA).
For respiratory secretions (tracheal & bronchoalveolar lavage), all specimens were inoculated for quantitative culture, if identified 10 – 10 CFU/ml, then gram staining was done followed by conventional biochemical test to identify specific gram negative organisms.
In Figure 14, volume renderings of a freshwater bryozoan (Cristatella mucedo) show the spatial relationships among all the structures in the specimen, with the potential for quantitative measurements and extraction of soft-tissue characters important for systematics [ 29].
Normal and tumor specimens from patients used for quantitative real-time RT-PCR analysis were obtained from the frozen tissue bank of the Erasmus Medical Center (Rotterdam, the Netherlands).
Specimens were next measured for quantitative protein expression with a DAB filter.
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