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This loose apposition is due to a lack of membrane interaction and the osmotic effects which occur during processing of the specimen for electron microscopy (EM) and indicates that the sperm acrosome is probably not docked with the PM in control sperm.
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Electron microscopic study Specimens for electron microscopic examination were immediately fixed in 2.5% glutaraldehyde buffered with 0.1 M phosphate buffer at pH 7.4 and then post-fixed in 1% osmium tetroxide in the same buffer.
Specimens for electron cryomicroscopy were prepared by the back-injection method in 4% trehalose (Breyton et al., 2002).
Electron Microscopy and Image Processing Specimens for electron cryomicroscopy were prepared by the back-injection method in 4% trehalose (Breyton et al., 2002 ).
Specimens for electron microscopy were obtained from 12 site and age-matched physes, 6 non-affected cn/+and 6 affected cn/cn.
Doyle and Crocker developed a series of methods that, in the case of synovial fluids, allows the rapid preparation of fresh specimens for electron microscopic examination, a procedure that previously took up to 48 h.
The physical principles of electron specimen interaction govern the design of specimen supports for electron cryomicroscopy (cryo-EM).
In addition, improved methods of specimen preparation for electron microscopy, combined with tomography, provide new opportunities for understanding Golgi structure.
Needle-shaped specimens fabricated for electron tomography need to meet specific requirements, often more strictly than for other applications as atom probe tomography, such as reduced needle diameter and minimized surface amorphous layer.
All specimens for transmission electron microscopy were examined with a JEM-1230 electron microscope (JEOL).
Plan view specimens for transmission electron microscopy (TEM) analysis were prepared by mechanically grinding away the backing silicon from the film.
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