In one Vulpes vulpes specimen, classification was of 1.436 below our classification threshold for modern mammal (2.0); in another Vulpes vulpes specimen, lack of classification was due to a low quality of the MALDI-TOF MS spectra (Table S5).
For specimen classification we used the Nearest Neighbour (NN) classifier, a statistical multi-factorial analysis tool known to perform well specifically for cancer classification when compared with other methods.
Specimen classification was made from 1 (quartile of the allograft-native bone contact zone) to 4 (quartile of the allograft surface), taking into consideration the most superficial cross-sectional subsection where new bone was present.
The material was collected and identified by Mona Ellithey, co-author of this article, using Red sea invertebrates' reference guide [ 19] which also provided detailed information specimen classification as indicated per organism below.
In renal biopsy specimen, histopathologic classification, scoring and grading system for renal amyloidosis was proposed [ 12].
In 1917 patients with reference pathologist assessment of both diagnostic biopsy and resected specimen, the classification was different for 75 (4%) patients.
In the surgical specimen, the classification of histological response proposed by Sinn et al. [ 22] was used (grade 0: no effect; grade I: resorption and tumour sclerosis; grade II: minimal focal invasive residues of ≤ 5 mm; grade III: only non-invasive tumour residues; grade IV: no viable tumour cell detectable).
To assess whether use of a single voided specimen for classification of proteinuria was sufficiently representative, we estimated the rate of persistent proteinuria in patients with micro- or macroalbuminuria and at least one additional albumin excretion measurement during the time window of this cohort.
However, it is not easy to obtain complete pathologic specimens, pathologic classification, and TNM staging after HIFU ablation.
The CAP checklists' content was supplemented with the most updated classification scheme (WHO classification), specimen details, staging as well as information on various ancillary techniques such as cytochemical studies, immunophenotyping, cytogenetics including Fluorescent In-situ Hybridization (FISH) studies and genotyping.
