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A specimen cell was held with two glass micropipettes, the tips of which were coated with a urethane resin adhesive.
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For SEM analysis, specimens cells were fixed in 2% gluteraldehyde in 0.1 M cacodylate buffer (pH 7.4).
Measurements on ten separate cells were made in each specimen, and each cell was subjected to three penetrations of the probe into the cell.
Differences between herpes virus recovery by culture and nPCR in relation to the specimen cell content was compared using the Fisher exact test.
For each specimen, 50 100 cells were enumerated with respect to the number of fluorescent signals of each probe.
In each specimen, positive cells were expressed as the mean number of cells/high power field, calculated in a minimum of 3 high power fields (defined as a magnification ×400).
In the same specimen, a second glial cell is labelled within the VNC.
The specimens set into the EIC cell were insulated from rod and grip by a surface oxidized zirconium tube.
In all tumor specimens, cancer cells were strongly positive for C5b-9.
In parallel experiments, OA cartilage specimens and cells were examined by cryoimmuno-EM with the same antibodies.
Tissue specimens and cells were homogenized, and the total cellular protein was extracted using the RIPA Lysis Buffer (Beyotime, Jiangsu, China), according to the manufacturer's instructions.
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