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For each specimen, at least 100 Mks were evaluated.
In order to verify the authenticity of the sequences obtained, DNA was sampled and sequenced for every specimen at least twice.
We analyzed each specimen at least 2× by using PCR, with the inclusion of positive and negative controls in each run.
One part of the liver specimen, at least 2 cm long, was used for diagnostic purposes while the remainder was immediately frozen in liquid nitrogen and stored at −80°C.
To calculate the locus-specific copy number changes in each case, we divided the copy numbers of each probe by the mean count of centromere 2. For each specimen, at least 100 cells were scored wherever possible.
In cases where the procedure was performed for malignant disease, normal breast tissue was obtained from a part of the specimen at least 3 cm from the malignant disease.
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A total of 400 grid openings were scanned for each lung specimen on at least four separate grids at magnifications of either 10,000 or 20,000.
Specimens (at least 1.6 cm length and 1.5 mm thick) were fixed in 10% neutral formalin for evaluation and embedded in paraffin for histological examination.
Still, I find it surprising that there's no regulation governing the treatment of invertebrate research specimens, at least not in this country.
As a result, in 2010, a team led by Svante Pääbo, at the Max Planck Institute for Evolutionary Anthropology in Germany, revealed it had sequenced the entire genome of a Neanderthal based on three specimens at least 38,000 years old from Vindija Cave, Croatia.
For a 160 mm thick sidewalk sample, the average carbonation was 28% for all interior specimens at least 20 mm from surfaces.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com