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We assessed 50 specimens for adequacy based on tumour cell content, size of the specimen and tissue consistency, and rejected 41 specimens based on these criteria, mostly due to low or no tumour cell content.
Newly included specimens were collected on cruise and field expeditions, from collaborators, or from the University of Louisiana at Lafayette Zoological (ULLZ) collection of molecular grade specimen and tissue samples (Table 1).
While it might not be necessary to routinely obtain DNA sequence from every interception, we recommend that the specimens should be treated (tissue sampling, specimen preservation) and stored (voucher specimen and tissue sample collections) in a manner that enables future genetic (e.g. mtDNA data) and morphological analysis of the detections.
Here, well-curated specimen and tissue collections have repeatedly been shown to be of great value for detecting and documenting contemporary phenotypic (Suarez and Tsutsui 2004; Carroll et al. 2005; Kitano et al. 2008) and genetic changes (Wandeler et al. 2007).
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But even more crippling was the destruction or contamination of vast quantities of laboratory specimens and tissue, bacterial and chemical samples.
In future studies, it would also be worthwhile to comprehensively evaluate mucosal specimens, and tissue- or lymphatic tissue-resident lymphocytes.
Voucher specimens and tissue samples were preserved in absolute ethanol and were frozen at −20°C or stored at 4°C.
In the past decade, several strategic research initiatives have focused on providing access to small repositories of tissue specimens and tissue related data to researchers across networks of institutions [ 4- 9].
Additionally, the long-term storage of (type) specimens in desiccating preservatives such as ethanol results in changes to specimen colour and tissue shrinking, which makes morphological comparison difficult.
Mean specimen length and tissue fragmentation were significantly higher in the BL groups than UG ones (P <.001).
Specimen collection and tissue analyses were approved by the Bologna University Ethical Committee on human tissues research.
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