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Protein spots that were differentially expressed in tissue specimen and cell line were marked.
Four punches from each specimen and cell pellet controls were placed on separate blocks as previously detailed [ 17, 18].
However, the primary specimen and cell line displayed very similar immunophenotypes and ultrastructural characteristics [ 20] suggesting that the cell line has not diverged greatly from that of the primary tumor.
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hMSC were cultured for 8 days on NiTi specimens, and cell viability was analyzed using two-color fluorescence staining.
Cytogenetic analysis was performed on metaphase spreads obtained from tumor biopsy or bone marrow specimens and cell lines.
BORIS and CTCF mRNA levels were measured in breast tissue specimens and cell lines using the Platinum® Quantitative PCR Supermix (Invitrogen).
Genomic DNA of all tumor specimens and cell lines was extracted using Gentra Pure Gene Tissue Kit (Qiagen) followed by DNA quantification using Picogreen dsDNA reagents (Invitrogen).
On the basis of a high constitutive RANK expression in breast cancer specimens and cell lines, recent data suggest that the RANK status in cancer cells determines their tendency to metastasize to bone whereas RANKL is abundantly expressed [16].
ZZ and AL extracted DNA and RNA samples from some breast tissue specimens and cell lines.
Hence, the present study comprehensively addressed these questions in esophageal tissues specimens and cell lines.
Primary medulloblastoma specimens and cell lines displayed variable p53 protein levels.
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