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We determined the fibroblast density index by adjusting the number of cells counted to the proportion of tumour fibrosis observed in the specimen (adapted from Erkan et al, 2008).
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Part (c) was then immersed in OCT freezing medium (Bayer Diagnostics) (correctly oriented in a mould), snap-frozen in liquid nitrogen and stored at −80 C before being cut into 5 um sections for immunohistochemistry (preparation of frozen specimens adapted from Slayden et al, 1995).
Whether this was truly visible on his specimens or adapted from knowledge of extant stomatopods remains unclear.
Evaluation was done by scoring specimens from 0 to 8 (adapted from the Ashcroft scale for pulmonary fibrosis) to determine the amount of product present in the specimens corresponding to each of the time frames.
A specific procedure was applied for museum specimens (protocol II [ 44] adapted from [ 86]).
Specimens were tested by kinetic ELISA (kELISA) adapted from an assay previously described for influenza [29].
Hazy fields of color, sprinkled with runes adapted from Shang-era oracle-bone script, are wonderful specimens of fifties informel abstraction.
All four specimens were stained using an ethanol-based phosphotungstic acid (PTA) solution, which was adapted from protocols described previously [ 3].
These serum specimens were collected from febrile volunteers in Kathmandu and Bharatpur and tested for WNV using a nested RT-PCR adapted from methods previously reported [ 7, 8].
Adapted from "How to Cook Everything".
Serves 6. Adapted from Ed Schoenfeld.
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