After 24 hr, medium was changed as specified and subsequently refreshed every other day.
In the short germ band mode of development, only the anterior-most segments are initially specified and subsequently the posterior segments are added sequentially from unsegmented posterior tissue, which is often called a posterior growth zone [ 72, 73].
The grade and quality are left open, to be subsequently specified when the customer desires a delivery, as below stated.
Participants were instructed to press the spacebar as soon as they recognized the number, which they subsequently specified by means of the designated key on the keyboard, followed by the "return" button.
The simultaneously estimated models' fit provided the baseline value against which subsequently specified models were compared.
This assumption is valid for RNA-based retrotransposons but makes the model less applicable to DNA-based transposons that undergo excisions resulting in descendants of the specified ancestor that subsequently lack the insertion.
These data suggest that the evolution of modern Drosophilids included an enlargement of these complexes, notably a multiplication of the genes in the E spl -C that sE spl -Cave subsequenthatseemified toeir roles in Notchavegnalling pathway.
Deep cortical layers (layers VI and V) are subsequently specified from E12.5 to E13.5, with production of superficial cortical layers (layers II, III, IV) predominating from E14.5 (Angevine and Sidman, 1961; Caviness, 1982; Gillies and Price, 1993; Levers et al., 2001; Price et al., 1997; Takahashi et al., 1999).
Subsequently, we specified a three-factor model assuming a single factor for each latent ability of laboratory task (RA, DM, and LT).
The cells were treated with indicated compounds (with media changes every 48 h) for the specified time, and were subsequently harvested for tissue culture experiments.
Bacteria isolates were first typed by Gram staining, and then inoculated onto 0.85% saline solution at a specified optical density and subsequently inoculated onto 96-well Biolog MicroPlates (Biolog, Inc, Hayward, USA).
