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Unless otherwise specified staining score is presented as the mean value of the 4 tumour or 2 adjacent normal samples from each patient.
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The cells were treated as specified, stained with 0.05 μg/ml rhodamine 123 for 1 h, and harvested by trysinization.
We further specified the staining to the medial portion of the ganglion.
Chang et al. show high PCNA positivity in FLs with positivity in both follicular and interfollicular areas without specifying the staining intensity[17].
Otherwise, a nucleolar pattern is usually defined by the positive staining of nucleoli but has to be specified by further staining of the chromatin region.
For the evaluation of the Ep-CAM staining we used standardized criteria to evaluate membranous staining specified by Dako for interpretation of the HercepTest for p185HER2 expression.
Reddish-brown coloring of the cytoplasm/nucleus of the follicles was specified as positive staining.
We confirmed that DT migration defects in hairy mutant embryos were not due to a failure to specify DT identity by staining for the DT marker, spalt; in both h22 and h674 mutant embryos, spalt was expressed in the same temporal and spatial manner as heterozygous siblings (data not shown).
Haematoxylin solution (Fluka, Buchs, Switzerland) were used for nuclear staining in specified cases.
Simply select from menu options to specify species, tissue type, and staining method, and the Dissect-O-Matic will take it from there!
Cells were stained with the specified antibodies in 100 µl of PBS containing 0.5% BSA (FACS buffer).
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