Sentence examples for specified stages from inspiring English sources

Wild-type and mutant embryos were produced by pair mating, the embryos were then collected for RNA isolation and fixed for in situ hybridization experiments at the specified stages.

For cell proliferation analysis, 5-bromo-2′-deoxyuridine (BrdU, 30 mg/kg of mouse) was injected IP twice daily for five days at the specified stages after birth (day 1 to 5), three weeks (21 days to 26 days), eight weeks (56 days to 61 days), twelve weeks (84 days to 89 days) and early-mid pregnancy (day 5 to day 12).

We found only subtle (tough still detectable) differences in endopolyploidy levels (EI) of the floral organs between specified stages of the flower development.

Embryos were allowed to develop to specified stages, harvested in phosphate buffered saline (PBS), monitored for GFP fluorescence and fixed in paraformaldehyde (PFA, 4% in PBS).

For examination of Notch target expression, total RNA was isolated from wild-type and mutant fins and larvae at the specified stages using TRIZOL according to standard protocols.

These data are supplemented with estimates of their annotation and relative expression levels at specified stages of ingrowth wall construction along with noting whether their homologues have been detected in transcriptomes of BETCs and nematode giant TCs (Additional file 5).

Equation 5a is used to determine the stage design strength ratio as a percentage using the specified stage periods.

The stage design strength ratio as a percentage is determined using Eq. 1 based on the difference between the design relative strength, S(T), estimated for a given design load applications level, W(T), and the stage relative strength, S t), associated with a specified stage load applications value, W t), that is less than or equal to the design load applications level, W(T).

At stage 21, about 12 hours after the onset of Shh expression in the wing bud, all three digits appear to have been specified [5]; stages 25 and 27 are about 24 and 48 hours later respectively.

The resulting L1 nematodes were fed and grown at 20°C to the specified developmental stage.

Color-coded arrows highlight the period of the specified developmental stage.