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To investigate the effects of various parameters to obtain the highest surface area, 27 experiments were carried out at various conditions with the changes of two parameters while the other parameter was kept constant at a specified medium value (PVA of 2.5 wt%, 50 wt% slurry solid content and inlet temperature of 200 °C).
For Figs. 13, HS NB, DSM 5820, DSM 5821, FBS NLP, and HS NLP reaching comparable densities (as judged by a turbidity of about 0.6 measured by A650 as well as by the size of pellets) were obtained from the indicated cultures and subcultured 1∶5 into the specified medium in 25 ml T-flasks.
Directly after transfection, cells were transferred to the previously specified medium and stimulated 6 h after transfection.
Cells were then treated for 20 minutes in 10% CO2 at 37°C in 50% Differentiation Medium (DMEM + 2%HS + 1% penicillin streptomycin) and 50% specified medium.
Given the biomass growth rate μ and the substrate uptake rates v C defined by the metabolic network, the population growth model provides a mechanism for calculating cell [ X] and substrate [ C] concentrations in the specified medium.
The most common method for separation, both during expansion seed trains and final product harvest stages, is to pellet the cells by dead-end (batch) centrifugation and then to resuspend the cells into a specified medium.
Similar(43)
Our waiter suggested we specify medium "next time".
Cohen's d ranged from 0.39 to 0.71, specifying medium to medium-large effect sizes for these scales (Cohen, 1992; Kotrlik and Williams, 2003).
Cohen's d as a measure of effect size for scales with significant differences ranged from 0.51 to 0.9, specifying medium-to-large effect sizes (Cohen, 1992).
The P. denitrificans ATCC 13867 strain was grown in M9 minimal medium containing 5 g/L sodium gluconate; the other microorganism strains listed in Table 1 were grown in a specified nutrient medium.
The Commission recognizes that this specified impact medium is the equivalent of, and will yield the same impact test results as, a surface covered with vinyl-asbestos tile meeting the requirements of Federal Specification SS-T-312A. 1 See footnote 1 to § 1500.51.
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