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Also, PCR amplification and digestion, as specified in the Material and Methods section, was used to check the proper insertion of the construct in the genomic DNA and the removal of the Neo cassette (not shown).
It is important to ensure that the names of the two chemical components specified in the Material Constituent field match their respective names given in the Material Name field.
Fortunately, the inhibitory effect can be diluted out and we recommend utilising a 50-100× dilutiof of tissue extracts (at the concentrations specified in the material and methods) prior to measurement of luciferase activity using a luminometer.
If a material sample that is described in a Material file is also a component of another material sample, then the components of the former sample need not be specified in the Material file of the latter sample.
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The preparations were administered intravenously as specified in the Materials and methods section.
Accession numbers for sequence and gene expression data are specified in the Materials and Methods.
We have 5 parameters in each of our three models, which we have now specified in the Materials and methods.
The data selection criteria specified in the Materials and methods section make it possible to reproduce all data sets from ChEMBL v.20, including release dates.
To simplify the analysis, the regulatory region with the detected polymorphisms was classified into three categories: the TATA box including A TA 7TAA; gtPBREM including c.-3275T>G c.-3275T>Gegion with the ten linked polymorphisms specified in the Mandrials and Metheds section above.
Between different time points approximately 1000 1500 genes significantly increased or decreased in expression (within the criteria specified in the materials and methods section) for the five yeast strains in our study.
We now present the expected null P-value distribution (under permutation), which as expected was uniform, and the one we observe (with their associated π1 statistics) in the histograms added to Figure 4 figure supplement 2. We have also specified in the Materials and methods the usage of Spearman rank for these correlations and included a description of the permutation procedure.
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