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Pre-cleared cell extracts were loaded on sucrose gradients and subjected to ultracentrifugation as specified in Materials and Methods.
Biochemical data were measured as specified in materials and methods.
Primers and probes were selected according to a series of criteria as specified in Materials and Methods.
The foreskin samples were cut in half: one half was used to establish fibroblast cultures, and the other half was used to establish SKP cultures, as specified in Materials and Methods (Toma et al., 2005).
In comparison, if grouped by traditional risk parameters (as specified in Materials and Methods), 77% of the patients would have been categorised as high risk with a 5-year RF-CSS of 78% and only 23% would have been categorised as low risk with a 5-year RF-CSS of 87%.
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Improvements in indicators of procedure and result are specified in "Material and methods".
Accession numbers for sequence and gene expression data are specified in the Materials and Methods.
The preparations were administered intravenously as specified in the Materials and methods section.
We have 5 parameters in each of our three models, which we have now specified in the Materials and methods.
The data selection criteria specified in the Materials and methods section make it possible to reproduce all data sets from ChEMBL v.20, including release dates.
Between different time points approximately 1000 1500 genes significantly increased or decreased in expression (within the criteria specified in the materials and methods section) for the five yeast strains in our study.
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