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On specified days of co-culture, cells were recovered from the culture dishes for analysis and sorting on a flow cytometer.
During the entire study period, subjects provided almost daily overnight urine samples and blood specimens during specified days of their menstrual cycles.
Some clinics offer dedicated sessions for hypertension and its complications on specified days of the week, designed to allow more time for detailed assessments and patient education.
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All these parameters were measured with the fifth rosette leaves, which were harvested at specified days counted from the day of leaf emergence.
Blood samples (300 μl) were collected from mice tail veins on the specified days, in the presence of heparin, and the plasma was further separated by centrifugation (1200 r.p.m. for 10 min).
Therefore, the expected lifetime of the proposed routing protocol on specified days depends on the event of the arrival rates and can be described as the energy that is needed to detect or sense the events continuously within a day, as shown by Eq. 16 [24].
Mr. Vitale sent 8,000 e-mail messages asking people to buy his book, "Spiritual Marketing: A Proven Five-Step Formula for Easily Creating Wealth from the Inside Out," from Amazon.com on one of two specified days last June; in return he offered them access to marketing articles posted at his Web site that normally were not available without a fee.
The fluorescence images were then recorded on specified days at the emission wavelength of the dye incorporated into the constructs.
But these deals are only available on a limited number of seats on specified days.
For experiments involving flow cytometric analyses, mice were killed on specified days after SEB challenge and distribution of CD4+ and CD8+ T cells expressing TCR Vβ6 or TCR Vβ8 was determined in indicated tissues by flow cytometry using flurochrome conjugated antibodies (BD biosciences (San Jose, CA).
On the specified day after infection, mice were challenged with 5000 PFU of the PR8 strain of influenza.
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