Exact(4)
Human embryonic stem cells (hESCs) harbor the capacity to differentiate into all primary human cell types [1], [2], [3] and can be cultivated in vitro indefinitely under specified culture conditions [4].
At the specified culture time point, mycelia were harvested, washed with distilled water, frozen in liquid N2 and then ground using a mortar and pestle.
At a specified culture period, such as the 6th day, mycelia in the culture medium were collected by filtration and rinsed with pH 2 water (50 ml) to scour off the residual nutrients as well as nonionic surfactants.
For biological assays, N2a cells at 60 80% confluence were plated out in specified culture plates at a density of 10 10 cells per well.
Similar(56)
Unless otherwise specified, cultures were grown at 25°C.
This in accord with the United Nations Agenda 21, which specifies culture as the fourth domain of sustainable development.
Based on these concepts, gender order may be defined as the different characters, roles, and modes of action for men and women that are specified by culture, customs, political economy, and the social structure, including social policy.
Unless otherwise specified, all culture reagents were from Invitrogen, UK.
According to the values, assumptions, and underlying beliefs that characterise a given unit, distinct types of culture can be specified (for example, clan culture, market culture, constructive culture).
To migrate cultures, the specified percentage of culture was removed from each of the three microcosms within a replicate to a common pool and mixed.
Transformants were confirmed by PCR using primers AD-BNI1-fseq/PTEFCH and AD-BNI1-rseq/pFa6 test (Table 4) and growth on YPD plates containing 300 μg/ml hygromycin B.>>> Unless otherwise specified, yeast cultures were grown in rich YPD medium supplemented with 0.3 mM adenine.
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