Eggan says his grants specified what cell lines he preferred to use, but also noted alternatives in case the guidelines didn't approve those lines.
Unless specifically specified, these cell lines were grown in the Dulbecco's Modified Eagle's Medium (DMEM) (Cellgro) or RPMI 1640 medium (Sigma) supplemented with 10% fetal bovine serum (FBS, Invitrogen).
The study was performed to evaluate the synergetic anticancer activity of Berberine and Curcumin by estimating the inhibition of the cell proliferation by cytotoxicity assay using MTT method on specified human cell lines (A549, Hep-G2, MCF-7, Jurkat, and K562).
In the literature, this mutation was also reported in a 'NOS' (not otherwise specified) mesothelioma cell line (Olivier et al, 2002).
Other regions specify cell envelope components.
A binding site is declared constitutive when its modal region contains peak centers from at least two replicate datasets per cell line for more than 90% (an arbitrary cutoff that the user can specify) of the available cell lines.
Notch receptor and ligand mutations in different cell lines, as specified in the CCLE-dataset.
Virus stocks were generated and used for transduction of various cell lines as specified in the Supplemental Experimental Procedures.
Antibodies, chemicals, oligonucleotides, plasmids and cell lines used are specified in Text S1.
President Bush said today that he would veto any legislation that tried to push federal support for embryonic stem cell research beyond the parameters he has specified, which confine public financing to stem cell "lines," or self-sustaining colonies, already derived from embryos.
