Exact(5)
Two positive culture definitions were used: ≥1 cfu/0.01 mL and ≥10 cfu/0.01 mL of the specified bacteria.
Unless specified, bacteria were cultured to mid-exponential (TSB, OD600 = 0.75; CCY and YCP, OD600 = 1.0) or stationary (TSB, CCY, and YCP, OD600 = 2.0) phases of growth.
The percentage of unidentified aetiology was 17.1%, the "other not specified bacteria" (ICD-9-CM code 320.7 without the specific code in primary or secondary diagnosis and codes 320.81 320.89) accounted for 12.9%.
The overall case fatality rate was 13%, the highest was from tuberculosis and other specified bacteria (both with 21%).
Nevertheless, not only the VFDB database is valuable for identifying genes linked to pathogenicity in specified bacteria (even strains), but it also serves as a benchmark for studies predicting pathogenicity linked gene clusters.
Similar(55)
Unless specified otherwise, bacteria were grown in trypticase soy broth (TSB; Becton Dickinson) in a flask-to-media volume ratio of 5∶1 at 225 rpm at 37°C.
Unless specified, extracellular bacteria were removed from the monolayers after a 2 h incubation by washing with pre-warmed RPMI+ medium containing 6 µg/ml of gentamicin (Sigma-Aldrich).
A recent World Health Organisation (WHO) report on antimicrobial resistance (AMR) surveillance specified nine bacteria of international concern which are responsible for some of the most common infections in community and hospital settings [ 3].
NPV for predicting absence of a specified potentially pathogenic bacteria or predicting "any bacteria" was high enough to rule out bacteriuria.
In the present study the antimicrobial properties of electrospun PLGA and BG45S5/PLGA fibres were investigated using human oral bacteria (specified with mass spectrometry) incubated for up to 24 h.
Cmax/MIC and AUC24/MIC ratios were calculated for the label dose of pradofloxacin 3 mg/kg based on MIC90-values published by Schink et al (2013) [ 12] for the bacteria specified in the Veraflox SPC, i.e. Staphylococcus (pseud)intermedius, Escherichia coli and Pasteurella multocida (Table 5).
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