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This defines the coordinate frame within which the position of each hypothetical camera is specified, as described below.

The nucleotide substitution model and substitution rate prior for HVR were specified as described above for the demographic reconstruction, and the coalescent Bayesian skyline tree prior was used [ 79].

As a preprocessing step, we reduced the genotype space within each gene to the most promising 200 SNPs (at most) that had the highest canonical correlation test score (Ferreira and Purcell, 2009) with the metabolites; however, to prevent overfitting, the priors were specified as described above using the unpruned SNP set.

In this work, the identification of correlations in the joint posterior distributions for the rate of protein synthesis and degree of partial repression raises the question as to whether the model is best specified as described, or whether an alternative formulation might be more suited for inference in which the posterior distributions for the parameters would not be correlated.

Similar(56)

Cells were washed and permeabilized with 90% ice-cold methanol for 15 min: PBMCs were stained with directly conjugated antibodies against CD4 (RPA-T4), CD8 (RPA-T8), CD20 (L27) and phospho-epitopes (Becton-Dickinson; San Jose, CA, unless otherwise specified) as previously described [22](Table S2).

The non-neuronal tissue was removed under a dissection microscope, and nerves were cultured in Opti-MEM supplemented with 1% penicillin/streptomycin and with glutamax (both Invitrogen) in a humid atmosphere at 30 °C, 5% CO2 for the times specified, as previously described.

Spatial learning and memory were evaluated by the Morris Water Maze (MWM) test starting 14 days after TGI and specified treatments as described previously with modifications.

Blood was drawn from patients while fasting at the specified time points as described above.

The rest of the analyses (unless otherwise specified) were performed as described by Ntougias et al. [ 38].

During this study, a second, "optimized" linear model of BCI (linear BCI) was developed and a post hoc analysis of the prognostic performance of linear BCI was performed using the specified risk groups as described previously [ 9, 12].

Low frequency forced oscillatory respiratory mechanics, venous and arterial blood gas (VetScan i-STAT1 Handheld Analyzer with EG6+ cartridge, Abaxis, Union City, CA, USA) and effective lung volume were collected and registered at specified time points as described thereafter.

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