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Primers were designed based on a strain-specific RFLP marker, and their specificity was verified under qualitative and quantitative PCR conditions based on successful CRT1 amplification and absence of cross-reaction with genomic DNA from various rhizosphere strains.
Its specificity was verified by visualizing particular DRB1-specific SSCP bands under test.
Antibody specificity was verified with IP and MSL1-specific RNAi followed by Western blot analysis and ChIP assay.
IRIP paralog specificity was verified by cloning the PCR-product of the paralog specific primers pairs using a TOPO TA cloning kit (Invitrogen) and subsequent sequencing of five to ten clones.
Specificity was verified with DNA samples isolated from different lactic acid bacteria.
Primers ChiF and ChiR were designed and synthesized (Table 1), and the specificity was verified by using PCR amplification and sequencing.
The RPPs gene-qPCR we established had a detection limit of one gene copy per reaction while its specificity was verified by sequencing.
Sensitivity and repeatability were established, and specificity was verified by testing multiple isolates of EHV-5, as well as DNA from other equine herpesviruses.
Specificity was verified by melting curve analysis.
Primer specificity was verified via cloning of PCR product.
Specificity was verified by a single peak in melting curve analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com