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Specificity was confirmed with competition experiments.
Assay specificity was confirmed by capillary electrophoresis and DNA sequencing.
Amplicon specificity was confirmed by checking the melting curve at the completion of 40 cycles.
The qPCR specificity was confirmed by the sharpness of the peak appeared in dissociation curve analysis.
Nanoparticle-VCAM-1 targeting specificity was confirmed by in vitro binding and competition studies.
The recognition specificity was confirmed using 3 strains of E. coli which showed negligible binding.
Specificity was confirmed after 4 weeks of culture.
Antibody specificity was confirmed by parallel stainings without the primary antibody.
Specificity was confirmed by running QPCR products on a 1% agarose gel after each QPCR experiment.
Reactions were carried out in triplicate and primer specificity was confirmed by melting curve analysis.
The secondary antibody specificity was confirmed on cryostat sections done without primary antibody incubation (figure 4C).
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