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Specificity was ascertained by degrading the drug sample in alkaline medium.
Specificity was ascertained in two dogs (see Additional file 1: Figure S1, experimental procedures).
Reaction specificity was ascertained by performing the Melt® procedure (58 99°C, 1°C/5 s) at the end of the amplification protocol, according to the manufacturer's instructions.
Specificity was ascertained by treating positive control sections as described above but omitting the primary antibodies.
Staining specificity was ascertained using MCF-7 cell pellets (caspase-3 null) and staurosporine-treated HUVEC cells as negative and positive controls, respectively.
The efficiency of purification with each BRD was monitored by analyzing the fraction unbound to anti-Kac; specificity was ascertained by analyzing the samples incubated with the inhibitor -JQ1.
Similar(53)
The α1-subunit of PAF-AH isoform I has a strong preference for acetyl groups attached to the glycerol backbone of PAF [24], and this specificity is ascertained to the Leu48, Leu194 and Thr103 residues of the enzyme by forming contact with the methyl group of acetate [17].
Specificity of ChIP was ascertained when no amplification was obtained in the group where the antibody against histone H3 was omitted.
Specificity of binding was ascertained by competition with a 160-fold molar excess of unlabelled consensus oligonucleotides.
The specificity of the method was ascertained by analyzing the standard drug and sample.
The specificity of the method was ascertained by analyzing drug standard solution and samples of equivalent concentration (0.8 μg/ml).
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