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The questions used to diagnose migraine have 75.0%% sensitivity and 88.2%% specificity, verified by comparing the diagnoses from the survey with those of doctors obtained from an additional telephone interview.
Samples that do not meet desirable PCR quality and specificity verified by melting-curve dissociation analysis were excluded from the analyses.
The recombinant protein was injected into rabbits by Cocalico Biologicals, and antisera specificity verified by ELISA and Western blotting against tetherin.
Each primer set was designed using Primer3 [ 35] and specificity verified by NCBI Blast [ 36].
Candidate sequences were compared with cDNA sequences and their specificity verified in the non-redundant human DNA database using a BLAST algorithm [accession through NCBI].
Probes were designed from the highly conserved N-terminal area of each cDNA sequence and their gene specificity verified by subsequent cloning and sequencing.
Similar(53)
Its specificity was verified by visualizing particular DRB1-specific SSCP bands under test.
Antibody specificity was verified with IP and MSL1-specific RNAi followed by Western blot analysis and ChIP assay.
Phospho-specificity of the antibody was confirmed using lambda phosphatase pre-treatment of tissue sections, and sequence specificity was verified using peptide blocking.
Specificity was verified by melting curve analysis and agarose gel electrophoresis.
Specificity was verified with DNA samples isolated from different lactic acid bacteria.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com