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This creates a major problem in the production and specificity verification of diagnostic anti-Ig reagents for the vast majority of mammals.
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There is a prospective clinical trial in Pusan National University Hospital, South Korea where combined ultrasonography methods using trans-cricothyroid membrane ultrasonography and ultrasonography lung-sliding assessment were found to have 100% sensitivity and specificity in verification of tube placement in emergency settings [15].
For cardiac and hypertension patients we observe decreased sensitivity and increased specificity for verification.
Although only one study provided data on specificity, partial verification bias was avoided in 64% of the studies.
This study was designed to examine whether a handheld, battery-operated fiberoptic bronchoscope (FOB) used to verify endotracheal tube (ETT) placement would be as sensitive and specific as other modes and whether a combination of multiple modes would further enhance the sensitivity and specificity of ETT placement verification.
This result and the minor expression patterns observed in MSP1 seedlings highlights the need for experimental verification of specificity prior to further practical analyses.
Moreover, the SYBR®Green chemistry is less expensive than TaqMan® and allows a post-amplification verification of specificity of the amplicon (Postollec et al. 2011).
Subsets of molecules (sequences) that were present in either L3 (n = 10) or xL3 (n = 10) or in both stages (n = 10) were selected for subsequent experimental verification of specificity by rtPCR.
Following the final selection of terms, in order to improve specificity, we performed random selection verification tests to examine the reliability of each term.
Please see Additional file 2 for a detailed description of the verification of antibody specificity and loading controls.
Verification of assay specificity was performed by using genomic DNA from a range of other bacterial species.
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