For both studies, no detailed interpretation was described for the specificity variations across different array designs and sample types.
To date, cytokeratins (CKs) have become the most widely accepted protein markers for the detection of epithelial tumor cells in mesenchymal tissues such as BM, blood or lymph nodes [ 8- 10]; however, different staining techniques can result in specificity variations [ 11, 12].
The quality of our microfluidic oDNA microarray including sensitivity, signal noise, specificity, spot variation and accuracy was characterized.
The sensitivity and specificity of variation detection of HRM are estimated, respectively, to be 100 and 95%[19]].
We explored trade-offs between sensitivity and specificity with variation in blood pressure thresholds for home and clinic measurements (table 8).
The envelope (Env) protein, expressed on the surface of virus particles, determines target cell specificity; thus, variations in target cell populations are likely to exert selection pressure on the virus.
However, if experimental specificities provide variation in the mean-max ratio, the cut-off will follow this variation.
Additional information such as sequence signatures, gene neighbourhood, domain architectures, interacting partners, conserved catalytic triads or motifs, functionally conserved active site residues, organism specificity, co-variation across metagenomes and interacting ligands can add confidence to function predictions [ 50, 53].
There are multiple isoforms of each subunit with tissue and species specificities, and variations among the digitalis sensitivities of the isoforms [ 6– 8].
In developing this protocol to amplify the EIV eight-segments, a series of processes, including phylogenetic analysis based on different influenza hosts, in silico analyses to estimate primer specificity, coverage, and variation scores, and investigation of host-specific amino acids, were progressively conducted to reduce or eliminate the negative factors that might affect PCR amplification.
