Sentence examples for specificity validation from inspiring English sources

In order to ascertain that the observed signals were not originating from the annealing of random non-specific sequences, specificity validation was conducted through a simple detection test using species- and gene-specific primers and by gene-specific microarray hybridization.

In addition to the variants validated to determine specificity, validation was also targeted to several hundred substitutions involved in regions of hypermutation and dinucleotides (Table S1 B).

These include the consideration of all gene splice variants to enable either gene-specific (covering the majority of splice variants) or transcript-specific (covering one splice variant) expression profiling, primer specificity validation, automated best primer pair selection according to strict criteria and graphical visualization of the latter primer pairs within their genomic context.

Potential research needs with respect to probe design and specificity, validation, sample concentration, selective target enrichment and amplification, and data analysis are discussed.

Although the assay showed 100% sensitivity and specificity, validation studies of further variants are required to reduce the CIs.

All primers were designed by using Primer Premier 5 (Premier Biosoft, Palo Alto, CA, USA) software with specificity validation.

In this case, an AUC value of 0,87 was reported with 83% sensitivity and 79% specificity, after validation in a cohort of 123 patients [204].

Although we did not independently verify case status in this study, discharge diagnoses of PD have been found to have high specificity in validation studies conducted by other investigators.

A 92% CIN 3 detection rate has been proposed as the clinical sensitivity along with an 85% CIN 3 confirmation as the clinical specificity for validation of all HPV assays [ 14].

Despite the identification of candidate proteins that have high diagnostic sensitivity and specificity in validation tests, translating these research findings to useful and reliable clinical tests still remains difficult (Zhang et al, 2004; Petricoin et al, 2002).

Although important, gene signatures have many issues as descriptors - for instance, loss of specificity in validation studies with an increased number of samples [ 10], generally poor cross-platform compatibility (Amsterdam and Rotterdam signatures virtually do not overlap in gene content), lack of mechanistic (functional) correlation with phenotype, etc.