We were able to evaluate and improve the experimental coverage of the serum glycome by a combination of (1) acidified PNGase F digestion, (2) increasing sample signal by sample processing improvements and gentle ionization conditions provided by a SPIN ionization source, and (3) using high-sensitivity, high-specificity software.
Test performance was evaluated by calculating sensitivity and specificity using SPSS software, version 12.0.1 (SPSS, Chicago, IL, USA).
The specificity of the software matches the best seen in literature for classifying medical text [ 13, 26].
To apply ExomeCNV we followed the instructions provided by the user guide, using 0.9999 as threshold for sensitivity and specificity, with the software set to optimize the specificity.
PolyPhen-2 predicts the pathogenicity of p.I202N to be "Probably Damaging" with a score of 0.968 (sensitivity: 0.77; specificity: 0.95); SIFT software predicted this mutation to be harmful, with a score of 0.03.
With further study and development, coupled with improved fidelity and granularity of data within the IW, it may be possible to increase the sensitivity and specificity of the software and to use this tool as one possible means of increasing the clinical trial recruitment rates.
Receiver-operating characteristic (ROC) analysis as well as comparisons of specificities of the software packages at similar levels of sensitivity therefore needs to be performed in order to compare the diagnostic performances.
The scope and specificity of the 112 software tool profiles is staggering - prices range from free solutions that team members can adopt individually to six-figure operating systems built for the enterprise.
With the DynaCAD software the specificity performance was analogous to that of the CADstream software.
The quality of the primers was tested by virtual PCR for dimerization and specificity using Amplify 3X software [ 86].
All primers were designed by using Primer Premier 5 (Premier Biosoft, Palo Alto, CA, USA) software with specificity validation.
