Trypsin (specificity set for cleavage after K or R) and V8 (specificity set for cleavage after D or E) were designated as proteases, and one missing cleavage was allowed.
We reached a sensitivity of 88% at a specificity set at 99%.
We considered four scenarios with varying prevalence (a) both sensitivity and specificity set at 95% (b) sensitivity of 70% and specificity of 95% (c) sensitivity of 95% and specificity of 70% and (d) both sensitivity and specificity set at 60%.
This low prediction sensitivity might be due to the high specificity set by the IScan program in identification of the TIR signals.
Computed WC and WHtR thresholds for specificity set at 75% were, respectively, 93 cm and 0.58 in women, and 83 cm and 0.50 in men.
The same model discriminated FIGO I + II patients from controls with a sensitivity of 74% at a specificity set at 99%.
The following search parameters were used: no restriction on protein molecular weight or isoelectric point, enzymatic specificity set to trypsin, and methionine oxidation allowed as a variable peptide modification.
An alternative algorithm with 65% specificity set in the second step was also evaluated, as was the application of the ESC/ERS guidelines to the DETECT population. 1 The performance measures of the decision tree and its internal validation using bootstrap methodology are described in detail in the online supplementary text and tables S8 S11.
In the second step (specificity set at 35%), the risk score from Step 1 was combined with echocardiographic tests to produce the final PAH risk prediction score to determine if a patient should be referred to RHC for diagnosis.
With these approaches substantial progress has been made in assessing receptor expression and determining ligand specificities, thus setting the stage for investigations on the mechanisms of OR signal transduction, which have yet to be resolved unequivocally [13], [14], [15].
