Exact(1)
Specificity of this experiment was supported by the observation that the mobility of the proteins in SDS-PAGE, which corresponded to molecular masses of 51 and 53 kDa, respectively, was in agreement with the calculated molecular masses of streptococcal α-enolase (47 kDa, GenBank accession no.: ZP_06612198) and GAPDH (36 kDa to 50 kDa; GenBank accession no.: BAD02480 and ZP_06612207).
Similar(59)
In practical terms, using low amounts of siRNA reduces the costs and increases the specificity of the experiment by avoiding non-specific knockdown of unrelated proteins and preventing the cell from undergoing a viral response.
To further investigate the performance of our system, trap efficiency and uniform ratio of microfluidic structure, and specificity of the experiment were evaluated and showed good consequences.
The transfection efficiency and specificity of the experiment were confirmed by analysing the input of LOX-1-myc and Sec8H4-myc in extracts before immunoprecipitation.
Specificity of the experiment was controlled by omitting the hydroxylamine treatment.
The endogenous production of hydroxyl radicals by PMNs is in doubt because the specificity of the experiments has been questioned, and it has been argued that exogenous iron in the buffers used during in vitro experiments could catalyze the production of hydroxyl radicals rather than endogenous production in PMNs [31].
To determine the specificity of this inhibition, parallel experiments were performed to determine whether oxaliplatin also modulates TNF secretion in activated RAW 264.7 cells.
To check for specificity of this assay, control experiments were run using probe sequences differing in a single-base substitution within the repeat monomer ((TTA CG n and (TTTA CG n).
To demonstrate the specificity of this response, we repeated this experiment in cells deficient for MAPK activation (ste11∆), in which the sensor remained enriched in the nucleus upon stimulation (Fig. 1d).
CNV regions (CNVRs) were determined by aggregating overlapping CNVs identified in different animals as previously reported [ 28, 51] and considering a conservative approach due to the specificity of our experiment (see Methods for details).
We also refer the reviewers to our previously published paper (Leinwand and Chalasani, 2013) where we used the same RNAi knockdown approach and tested the specificity of this approach with negative control experiments targeting different neurotransmission pathway genes and also targeting alternate amphid sensory neurons (using alternate cell-specific promoters).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com