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About one-third of the total clones are singletons, and approximately one-fourth of the ESTs fit in clusters that are comprised of more than 50 ESTs, representing the complexity and specificity of the transcript population of the venom gland of D. acutus.
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In order to evaluate the diversity and specificity of the transcripts that were specific to a physiological condition, individual library specific ESTs were studied.
In order to evaluate the specificity of the transcripts involved in both the developmental processes studied here, i.e. meiosis and follicle formation, individual library specific ESTs were studied.
The specificity of the transcripts most neutralised by sifalimumab administration was evaluated on a patient-by-patient level, using at least 50% neutralisation in the blood for each patient visit.
Yet, it is the absence of the maternal specific allele (e.g. G-allele in case 1160) that confirms the fetal-specificity of the transcript.
In analyses combining publicly available data, the remarkable specificity of these transcript abundances for DM is impressive.
Then 454 adapters A (5′-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3′) and B (5′-CTGAGACTGCCAAGGCACACAGGGGATAGG-3′) were ligated to the 5′ and 3′ ends of the cDNAs, respectively, to obtain strand-specificity of the transcripts.
Among the highly expressed top 50 Thitsithe the specificity of these transcripts relative to Th0, but not to Th1, has already been identified at different time points with the standard LIMMA methods (Smyth, [ 2005]) in the past [ 17].
To verify the specificity of these transcripts RT-PCR analysis was performed using cDNAs from pupa, larva, reproductive tract (ovaries, uterus, spermathica) and the remaining carcass mRNA from 10 adult flies at varying ages (Table 2).
Finally expression specificity of the assembled transcripts provided an accurate estimation of the biological processes involved in different tissues (Stigma or Flower).
RT-PCR confirms the female SG specificity of these transcripts.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com