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The specificity of the template needs to be balanced against the amount of data required in its construction and its ease of use in practice.
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The number of reads mapped to promoters and exons was compared for each library to confirm the cap-specificity of the template-switching reactions.
The remaining difference arises from differences in the specificity of the reaction templates.
HPV 16-specific primers were tested using PCR and the HPV 16 DNA template (Lane 4) to confirm the band size and specificity of the primers to the template.
The binding specificity of the primer with the template was controlled by ACP primers which consisted of UP primer at the 5′ end, intermediate [poly dI)] linker, and LAD primers of hiTAIL-PCR at the 3′ end.
The steady-state and time-resolved fluorescence spectroscopy studies are presented as methods for monitoring of the specificity and selectivity of binding of the template on the functionalised cavities.
The use of an internal competitor to obtain increased specificity of target:template detection demonstrate data for these time periods and is highly consistent.
Classical batch rebinding experiments demonstrated the specificity of the MIP towards its original template histamine.
In our opinion the really astonishing feature is the notable template specificity of the probe that leads to the observation of a detectable τD component in the decays obtained with genomic DNA (even when non-purified), which prevails in all cases.
Amplification products were visualized on 1% agarose gels to verify the specificity of probe-template binding.
Amplification products can be visualized on 1% agarose gel to verify the specificity of probe-template binding or directly by adding fluorescent dyes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com