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Other explanations for the discrepancies in microchimerism results are differences in experimental designs and the sensitivity and specificity of the techniques used by different investigators [18].
The absence of DAT expression was not due to a lack of specificity of the techniques used since SN neurons serving as positive control were clearly positive for DAT-IR (Fig. 8A), DAT binding (Fig. 8D) and DAT mRNA expression (Fig. 8G).
This important variability in incidence of tumour cell contamination in PBSC collections in previous reports might be due to different sensitivity and specificity of the techniques of detection, to different times of harvesting and to different disease status at the time of PBSC collection.
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However, there is inconclusive evidence in the peer-review literature on the specificity of these techniques in the evaluation of the effect of corneal cross-linking [ 8].
For a method that is used under routine conditions or for epidemiological studies, it is necessary to assess the diagnostic sensitivity and diagnostic specificity of the technique.
However, the introduction of SPECT/CT has led to a major improvement in the specificity of the technique and it has become a very valuable tool in the assessment of the painful knee arthroplasty (Hirschmann et al. 2013).
We demonstrate high quality of the isolated RNA from a defined cell population, the periglomerular cells of the olfactory bulb, and show the usefulness and specificity of the technique for subsequent gene expression studies by quantitative real-time PCR and microarrays studies.
This in turn may improve the specificity of the technique and minimise variation [ 15].
The vector specificity of the technique and its sensitivity to reflect levels of exposure in humans has not been addressed.
Moreover, the effectiveness and specificity of the technique depend upon eliminating highly repetitive DNA sequences from the painting probe.
Owing to the high specificity of the technique, the real-time PCR allows to distinguish human from mouse gene expression in xenograft models.
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