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The GMOMETHODS detection methods are currently based on the polymerase chain reaction (PCR) technology and are grouped according to the specificity of the target sequence: Event-specific methods have the highest levels of specificity, as they target regions that are unique to each DNA integration event.
A series of gene expression profiling experiments and quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) analyses were carried out to assess both the specificity of the target gene knockdown and the functional studies of the targeted signaling molecules.
All the drugs tested induced an increase of surface roughness, to an extent correlated with the localization and specificity of the target.
All the drugs induced substantial modifications of the cell surface architecture, to an extent related to the specificity of the target.
Structural characterization indicates the net-negative charge balance on the new designs enhances the specificity of the target state at the expense of its stability.
In this experiment, seven participants listened to 10 sentences of converted speech using the linear-based, the ARBM, and the SATBM approaches accompanied with the target speech and voted for the most preferable method for each sentence in terms of the speaker specificity of the target speaker.
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The success of this strategy is mainly due to the specificity of the target-specific primers, allowing to initially amplify the targets by PCR and, then, to enrich them by two successive semi-nested PCRs (Table 1).
The choice of 5′ non-homologous extensions in primer pairs used for amplifying the marker cassettes determines the site specificity of the targeting DNA.
The avidin-biotin system was also used for pretargeted radioimmunotherapy of lung cancer, but the lack of specificity of the targeting antibody resulted in severe toxicity (Knox et al. 2000).
The level of PAPST1 mRNA was unaffected by the PAPST2 siRNAs, and similarly the level of PAPST2 mRNA was not decreased by the PAPST1 siRNAs (Figure 2A), which confirmed the specificity of the targeting sequences.
In Section 2, we present our motivations for this research field and the specificities of the target applications.
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diversity of the target
uniqueness of the target
specification of the target
characteristics of the target
specificity of the targeting
specificity of the biosensor
specificity of the play
specificity of the library
specificity of the amplification
specificity of the stepfather
specificity of the aptamer
specificity of the sensor
specificity of the antibody
specificity of the bioelectrode
specificity of the genotyping
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