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Having observed that the sera exhibit at least some neutralizing activity, we wanted to assess the specificity of the serum antibodies for oligomannose.
The purity of the antigen and specificity of the serum is documented in Figure S1.
The antibody specificity of the serum was validated by ELISA and immunoblotting using rPEG11 as the antigen.
Titer and specificity of the serum was tested by ELISA and immunoblot blot as described previously [39].
To confirm the strength and specificity of the serum effect, a serum bactericidal assay was performed with different concentrations of serum.
The calculated specificity of the serum ELISA was therefore 100%.
Similar(48)
In this regard, a positive group and a negative group are indispensable to determine the sensitivity and specificity of either the serum or the CSF parameters in the differential diagnosis of meningitis.
The specificity of the factor serum 6 d was in agreement with a recent validation study of this factor serum performed by a different research group [ 14].
To test for specificity of the PfSUMO serum antibodies, we performed peptide competition assays and subsequent immunofluorescence.
Because scrub typhus has not been reported in Kenya, Western blot was performed to confirm specificity of the reactive serum samples, essentially as described before (5 ).
It suffers from poor specificity, and elevation of the serum PSA is often due to non-malignant causes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com